@article{oai:redcross.repo.nii.ac.jp:00014114,
 author = {頼田, 顕辞 and 黒田, 直人 and 吾妻, 美子 and 和田, 有加里 and 小原, 昌彦 and 安岡, 香 and 水野, 圭子 and 福田, 潤弥 and 中川, 英幸 and 宮崎, かつし and 谷田, 信行 and 浜口, 伸正},
 issue = {1},
 journal = {高知赤十字病院医学雑誌, Medical Journal of Kochi Red Cross Hospital},
 month = {Mar},
 note = {Objective: The MYB-NFIB fusion gene has recently been detected in adenoid cystic carcinomas (ACCs)
of the salivary gland and other organs. Detection of MYB rearrangement appears to be diagnostically
valuable for ACCs, particularly in high-grade transformed ACCs (HGT-ACCs). However, a consensus
cutoff value defining MYB rearrangement using fluorescence in situ hybridization (FISH) has not been
established. The aim of this study was to investigate an appropriate evaluation of MYB rearrangement in
ACCs using FISH.
Study Design: We retrospectively selected 10 ACC cases diagnosed between 2000 and 2015. FISH
analysis was performed using formalin-fixed paraffin-embedded sections and a MetaSystems image
analysis system.
Results: The involved sites were the major salivary gland (n=4), oral cavity (n=2), lip (n=1), nasal cavity
(n=1), external acoustic meatus (n=1), and trachea (n=1). One case was of HGT-ACC. Six cases (60%)
including the HGT-ACC showed positive MYB rearrangement, exhibiting over 85% split MYB signals.
The other cases were negative, demonstrating fewer than 10% split MYB signals, as observed in nonneoplastic
cells.
Conclusion: The MYB rearrangement cutoff value in ACC cells was 10%. MYB rearrangement
heterogeneity in ACC cells appeared to not be present. This genetic study supported the diagnosis of
HGT-ACC.},
 pages = {1--6},
 title = {Computer-aided automated evaluation of MYB rearrangement in 10 cases of adenoid cystic carcinoma using fluorescent in situ hybridization: a single-institution study(和訳中)},
 volume = {23},
 year = {2019}
}